RNA Sequencing

Beyond classic RNA expression analysis, RNA sequencing enables the analysis of structural information encoded in the transcriptome, such as splice variants, gene fusions, mutations and RNA editing events, many of these of paramount relevance for biomarker-based diagnostics and precision medicine.

RNA sequencing is the preferred method for a number of applications, including:

  • Gene signature discovery
  • Molecular characterization of tumors
  • Elucidation of disease mechanisms or discern patient subgroups
  • Study of drug mode of action

Biogazelle offers a complete solution for these applications - ranging from basic research to pre-clinical studies and clinical trials - through our expert RNA sequencing and data analyses services. Our services are applicable on human, mouse and rat samples.

small-RNA-sequencing“I convinced myself in the first telephone meeting that Biogazelle was at
the leading edge with their technology and science for cancer biology.”

Erik van Beers, VP science and technology at SkylineDx

Let's start

An RNA sequencing workflow for each project

Biogazelle offers a tailored sequencing workflow depending on your project needs. Check the below table to learn which RNA sequencing workflow best fits your application, RNA biotype or sample type.

Application RNA biotype Sample type Workflow
  microRNAs and other small RNAs
  • Fresh frozen tissue
  • Cells
  • Fixed tissue
  • Whole blood
  • Body fluids
Small RNA sequencing
Protein coding mRNAs
  • Fixed tissue
  • Body fluids
mRNA capture sequencing
  • Fresh frozen tissue
  • Cells
  • Whole blood
  • Stranded polyA+ RNA sequencing
  • 3' end sequencing
Protein coding mRNAs and polyadenylated lncRNAs
  • Fresh frozen tissue
  • Cells
  • Whole blood
Stranded total RNA sequencing
Protein coding mRNAs and all lncRNAs
  • Fresh frozen tissue
  • Cells
  • Whole blood
Stranded total RNA sequencing
  • Fixed tissue
  • Body fluids
mRNA + lncRNA capture sequencing
  • Transcript expression profiling
  • Fusion gene detection
  • Mutation detection
Protein coding mRNAs
  • Fixed tissue
  • Body fluids
mRNA capture sequencing
Protein coding mRNAs and polyadenylated lncRNAs
  • Fresh frozen tissue
  • Cells
  • Whole blood
Stranded polyA+ RNA sequencing
Protein coding mRNAs and all long non-coding RNAs
  • Fresh frozen tissue
  • Cells
  • Whole blood
Stranded total RNA sequencing
  • Fixed tissue
  • Body fluids
mRNA + lncRNA capture sequencing

Benefits of our RNA sequencing services

  • They include RNA extraction, library preparation, sequencing, data analysis and reporting
  • Are optimized for formalin-fixed paraffin embedded tissue (FFPE) and liquid biopsies (body fluids, e.g. plasma, serum, urine, cerebrospinal fluid etc.)
  • Require low amounts of precious patient material (100 ng of cellular RNA, 200 μl of biofluids)
  • We provide tailored sequencing workflows, aligned to specific research questions
  • We support different applications, including an end-to-end program from biomarker discovery to test development
  • Our IT infrastructure for RNA sequencing data can process >100 sample projects in a single day, helping you to meet tight timelines
  • Comprehensive data analysis capabilities tailored to your specific project design and requirements
  • Orthogonal result validation using quantitative / digital PCR technologies
  • Optional depletion of unwanted small RNA molecules from the total RNA
  • Most comprehensive human reference transcriptome for rich annotation, including Ensembl and LNCipedia
  • Project executed in an ISO17025 accredited and GCLP-compliant lab
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