RNA sequencing services

Beyond classic RNA expression analysis, RNA sequencing enables the analysis of structural information encoded in the transcriptome, such as splice variants, gene fusions, mutations and RNA editing events, many of these of paramount relevance for biomarker-based diagnostics and precision medicine.

RNA sequencing is the preferred method for a number of applications, including:

Gene signature discovery
Molecular characterization of tumors
Elucidation of disease mechanisms or discern patient subgroups
Study of drug mode of action

Biogazelle offers a complete solution for these applications - ranging from basic research to pre-clinical studies and clinical trials - through our expert RNA sequencing and data analyses services. Our services are applicable on human, mouse and rat samples.

“I convinced myself in the first telephone meeting that Biogazelle was at
the leading edge with their technology and science for cancer biology.”
Erik van Beers, VP science and technology at SkylineDx

An RNA sequencing workflow for each project

Biogazelle offers a tailored sequencing workflow depending on your project needs. Check the below table to learn which RNA sequencing workflow best fits your application, RNA biotype or sample type.

Application	RNA biotype	Sample type	Workflow
	microRNAs and other small RNAs	Fresh frozen tissue Cells Fixed tissue Whole blood Body fluids	Small RNA sequencing
	Protein coding mRNAs	Fixed tissue Body fluids	mRNA capture sequencing
	Protein coding mRNAs	Fresh frozen tissue Cells Whole blood	Stranded polyA+ RNA sequencing 3' end sequencing
	Protein coding mRNAs and polyadenylated lncRNAs	Fresh frozen tissue Cells Whole blood	Stranded total RNA sequencing
	Protein coding mRNAs and all lncRNAs	Fresh frozen tissue Cells Whole blood	Stranded total RNA sequencing
	Protein coding mRNAs and all lncRNAs	Fixed tissue Body fluids	mRNA + lncRNA capture sequencing
Transcript expression profiling Fusion gene detection Mutation detection	Protein coding mRNAs	Fixed tissue Body fluids	mRNA capture sequencing
	Protein coding mRNAs and polyadenylated lncRNAs	Fresh frozen tissue Cells Whole blood	Stranded polyA+ RNA sequencing
	Protein coding mRNAs and all long non-coding RNAs	Fresh frozen tissue Cells Whole blood	Stranded total RNA sequencing
	Protein coding mRNAs and all long non-coding RNAs	Fixed tissue Body fluids	mRNA + lncRNA capture sequencing

Benefits of our RNA sequencing services

They include RNA extraction, library preparation, sequencing, data analysis and reporting
Are optimized for formalin-fixed paraffin embedded tissue (FFPE) and liquid biopsies (body fluids, e.g. plasma, serum, urine, cerebrospinal fluid etc.)
Require low amounts of precious patient material (100 ng of cellular RNA, 200 μl of biofluids)
We provide tailored sequencing workflows, aligned to specific research questions
We support different applications, including an end-to-end program from biomarker discovery to test development
Our IT infrastructure for RNA sequencing data can process >100 sample projects in a single day, helping you to meet tight timelines
Comprehensive data analysis capabilities tailored to your specific project design and requirements
Orthogonal result validation using quantitative / digital PCR technologies
Optional depletion of unwanted small RNA molecules from the total RNA
Most comprehensive human reference transcriptome for rich annotation, including Ensembl and LNCipedia
Project executed in an ISO17025 accredited and GCLP-compliant lab

RNA Sequencing

An RNA sequencing workflow for each project

Benefits of our RNA sequencing services

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