- Knowledge center
This poster was presented at the NextGen Omics UK virtual meeting, November 2020
Since becoming commercially available in 2011, digital PCR (dPCR) has been gaining in popularity as it enables nucleic acid quantification with superior accuracy and precision compared to quantitative PCR (qPCR). In brief, the technology consists of the following steps:
With data acquisition taking place at the end of the reaction and since absolute concentrations can be determined, data can be easily interpreted and compared across different samples and experiments, especially in a clinical setting. Additionally, dPCR doesn’t suffer from the inherent complications of qPCR that result from the need of a standard curve, and excels in the quantification of low abundant genes or small differences.
In this poster, we perform a quick comparison on qPCR versus dPCR, and outline the intrinsic potential of ddPCR in clinical research for 3 different applications: