Identification of small non-coding RNAs in renal cell carcinoma using small RNA sequencing

Case Study

Renal cell carcinoma (RCC) is the most frequent renal malignancy. An increasing number of small renal tumors – often benign – is detected, due to the widespread use of abdominal ultrasonography for check-up or clarification of non-specific symptoms. Current modalities do not allow precise discrimination of malignant tumors, often leading to overtherapy. Thus, additional diagnostic parameters could help the clinician to improve patients treatment. However, to date, no biomarker is available for clinical practice, making an accurate and non-invasive identification of RCC impossible.

The team of prof. Ellinger set about to search for small non-coding RNA biomarkers. To this end, Biogazelle performed small RNA sequencing to determine the expression of small RNAs in RCC. In addition to microRNAs, 32 cleaved tRNA fragments, named tRNA halves, were found to be differentially expressed, and these findings were confirmed by RT-qPCR in an independent patient cohort. The team of prof. Ellinger concludes that 5′ cleavage of tRNAs occurs in RCC, but the exact functional implication of tRNA-halve deregulation remains to be clarified. They have published their findings in Scientific Reports.

"The interaction with Biogazelle was always excellent: The project was performed very quickly, communication clear and queries answered promptly. The service entirely met all our expectations and allowed successful completion of the project on small non coding RNA expression in renal cell carcinoma"

Prof. Jörg Ellinger, Klinik und Poliklinik für Urologie und Kinderurologie, Universitätsklinikum Bonn, Germany


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